Journal: BMC Plant Biology

Article Title: Impact of glyphosate and glyphosate-based herbicides on phyllospheric Methylobacterium

doi: 10.1186/s12870-024-04818-x

Figure Lengend Snippet: Inventory of Methylobacterium strains examined for glyphosate sensitivity

Article Snippet: Freeze-dried cultures of Methylobacterium spp . were obtained from four microbe collections: the Belgian Coordinated Collections of Microorganisms (BCCM/LMG), the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) [“German Collection of Microorganisms and Cell Cultures”], the Japan Collection of Microorganisms (JCM), and the National Institute of Technology and Evaluation’s (NITE) Biological Resource Center (NBRC).

Techniques: Isolation

Journal: BMC Plant Biology

Article Title: Impact of glyphosate and glyphosate-based herbicides on phyllospheric Methylobacterium

doi: 10.1186/s12870-024-04818-x

Figure Lengend Snippet: Average ( n = 3) zone of inhibition of each tested strain of Methylobacterium spp. (Table ) against maximum and minimum concentrations glyphosate (380 µg and 95 µg, respectively) in the WeatherMax ® [left] and the Transorb ® [right] products tested

Article Snippet: Freeze-dried cultures of Methylobacterium spp . were obtained from four microbe collections: the Belgian Coordinated Collections of Microorganisms (BCCM/LMG), the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) [“German Collection of Microorganisms and Cell Cultures”], the Japan Collection of Microorganisms (JCM), and the National Institute of Technology and Evaluation’s (NITE) Biological Resource Center (NBRC).

Techniques: Inhibition

Journal: BMC Plant Biology

Article Title: Impact of glyphosate and glyphosate-based herbicides on phyllospheric Methylobacterium

doi: 10.1186/s12870-024-04818-x

Figure Lengend Snippet: Representative photograph illustrating zone of inhibition of Methylobacterium gnaphali (NBRC 107716) to four concentrations of Transorb ® [left] and WeatherMax ® [right]

Article Snippet: Freeze-dried cultures of Methylobacterium spp . were obtained from four microbe collections: the Belgian Coordinated Collections of Microorganisms (BCCM/LMG), the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) [“German Collection of Microorganisms and Cell Cultures”], the Japan Collection of Microorganisms (JCM), and the National Institute of Technology and Evaluation’s (NITE) Biological Resource Center (NBRC).

Techniques: Inhibition

Journal: BMC Plant Biology

Article Title: Impact of glyphosate and glyphosate-based herbicides on phyllospheric Methylobacterium

doi: 10.1186/s12870-024-04818-x

Figure Lengend Snippet: Cell viability test ( n = 1) of each strain of Methylobacterium spp. against two concentrations of the Transorb ® (Tsorb) and WeatherMax ® (WMax) product formulations. Negative control involved nutrient-rich trypic soy broth (TSB) with no addition of commercial products

Article Snippet: Freeze-dried cultures of Methylobacterium spp . were obtained from four microbe collections: the Belgian Coordinated Collections of Microorganisms (BCCM/LMG), the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) [“German Collection of Microorganisms and Cell Cultures”], the Japan Collection of Microorganisms (JCM), and the National Institute of Technology and Evaluation’s (NITE) Biological Resource Center (NBRC).

Techniques: Negative Control, Control

Journal: BMC Plant Biology

Article Title: Impact of glyphosate and glyphosate-based herbicides on phyllospheric Methylobacterium

doi: 10.1186/s12870-024-04818-x

Figure Lengend Snippet: Cell viability test ( n = 2) of each strain of Methylobacterium spp. against two concentrations of pure glyphosate (GLY), with and without Tween20 (polysorbate-20). Negative control involved nutrient-rich trypic soy broth (TSB) with no addition of glyphosate or Tween20

Article Snippet: Freeze-dried cultures of Methylobacterium spp . were obtained from four microbe collections: the Belgian Coordinated Collections of Microorganisms (BCCM/LMG), the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) [“German Collection of Microorganisms and Cell Cultures”], the Japan Collection of Microorganisms (JCM), and the National Institute of Technology and Evaluation’s (NITE) Biological Resource Center (NBRC).

Techniques: Negative Control, Control

Journal: BMC Plant Biology

Article Title: Impact of glyphosate and glyphosate-based herbicides on phyllospheric Methylobacterium

doi: 10.1186/s12870-024-04818-x

Figure Lengend Snippet: Representative photograph illustrating results of cell viability test from cultures containing 0.1% pure glyphosate (1 mg/mL) with Methylobacterium gnaphali (NBRC 107716) with varying concentrations of Tween20 (polysorbate-20); ( A ) control, ( B ) 0.5%, ( C ) 1.0%, ( D ) 2.0%, ( E ) 4.0% (v/v). Frame ( A ) depicts confluent growth of NBRC 107716

Article Snippet: Freeze-dried cultures of Methylobacterium spp . were obtained from four microbe collections: the Belgian Coordinated Collections of Microorganisms (BCCM/LMG), the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) [“German Collection of Microorganisms and Cell Cultures”], the Japan Collection of Microorganisms (JCM), and the National Institute of Technology and Evaluation’s (NITE) Biological Resource Center (NBRC).

Techniques: Control

Journal: BMC Plant Biology

Article Title: Impact of glyphosate and glyphosate-based herbicides on phyllospheric Methylobacterium

doi: 10.1186/s12870-024-04818-x

Figure Lengend Snippet: Graphical representation of dry pellet weight of three distinct Methylobacterium strains when cultured in tryptic soy broth (TSB) containing a fixed quantity of pure glyphosate (0.1% w/v) in relation to changes in the presence of Tween20 (polysorbate-20), relative to controls containing Tween20 alone ( n = 4). The Student’s t-test was used to assess statistical difference between groups. A star (*) indicates statistical difference in pellet weight between control conditions (TSB only) and following the application of a treatment ( p < 0.05 = *, p < 0.01 = **, p < 0.001 = ***). A dagger (†) indicates statistical difference comparing pellet weight between the application of Tween20 and the corresponding application of Tween20 with the addition of glyphosate ( p < 0.05 = †, p < 0.01 = ††, p < 0.001 = †††)

Article Snippet: Freeze-dried cultures of Methylobacterium spp . were obtained from four microbe collections: the Belgian Coordinated Collections of Microorganisms (BCCM/LMG), the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) [“German Collection of Microorganisms and Cell Cultures”], the Japan Collection of Microorganisms (JCM), and the National Institute of Technology and Evaluation’s (NITE) Biological Resource Center (NBRC).

Techniques: Cell Culture, Control

Journal: BMC Plant Biology

Article Title: Impact of glyphosate and glyphosate-based herbicides on phyllospheric Methylobacterium

doi: 10.1186/s12870-024-04818-x

Figure Lengend Snippet: Graphical representation of average pellet fresh weight (line, right axis) of three distinct Methylobacterium strains when cultured in tryptic soy broth (TSB) containing fixed quantities of the active ingredient (AI), glyphosate, obtained from the Transorb ® commercial product, and corresponding intracellular formaldehyde concentrations (bar, left axis) after 4 days of growth at 27 o C ( n = 4)

Article Snippet: Freeze-dried cultures of Methylobacterium spp . were obtained from four microbe collections: the Belgian Coordinated Collections of Microorganisms (BCCM/LMG), the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) [“German Collection of Microorganisms and Cell Cultures”], the Japan Collection of Microorganisms (JCM), and the National Institute of Technology and Evaluation’s (NITE) Biological Resource Center (NBRC).

Techniques: Cell Culture

Journal: BMC Plant Biology

Article Title: Impact of glyphosate and glyphosate-based herbicides on phyllospheric Methylobacterium

doi: 10.1186/s12870-024-04818-x

Figure Lengend Snippet: Schematic illustrating basic metabolic pathways that may lead to increased intracellular formaldehyde load in Methylobacterium spp

Article Snippet: Freeze-dried cultures of Methylobacterium spp . were obtained from four microbe collections: the Belgian Coordinated Collections of Microorganisms (BCCM/LMG), the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) [“German Collection of Microorganisms and Cell Cultures”], the Japan Collection of Microorganisms (JCM), and the National Institute of Technology and Evaluation’s (NITE) Biological Resource Center (NBRC).

Techniques: